Use of Genomic in Situ Hybridization (GISH) to Track Genetic Introgression in Onion
نویسندگان
چکیده
منابع مشابه
Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora.
The genomic in situ hybridization (GISH) technique was applied to Passiflora interspecific F1 HD13-133 hybrids (Passiflora sublanceolata x Passiflora foetida) and HD15-101 (Passiflora gardineri x Passiflora gibertii), and the backcrossed hybrids (BC1) HD18-106 and HD18-113 (Passiflora sublanceolata x HD13-133). GISH was performed using genomic probes prepared with the DNA from the paternal geni...
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Fluorescence in situ hybridization was modified by using genomic DNAs as probes to detect wide cross somatic hybrids. The A genome was labelled and visualized as red signal while the B and C genomes were visualized as blue and green signals, respectively. This technique could distinguishly identify the hybrid SP I line with whole full sets of 6 genomes and the hybrid SP II line with some lost c...
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ژورنال
عنوان ژورنال: HortScience
سال: 1997
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.32.3.513b